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Erythrocytes |
Low Power Laser Protects Human
Erythrocytes In An In Vitro Model Of Artificial
Heart-
Lung Machines
Itoh T, Murakami H, Orihashi K, Sueda T, Kusumoto Y,
Kakehashi M, Matsuura Y.
Artif Organs. 2000 Nov;24(11):870-3.
First Department of Surgery Second Department of
Anatomy Institute of Health Sciences,
Hiroshima University School of Medicine, Hiroshima,
Japan. The protective effect of the low
power helium-neon (He-Ne) laser against the damage
of human erythrocytes in whole blood was
examined in a perfusion model using an artificial
heart-lung machine. Preserved human whole
blood was diluted and perfused in 2 closed circuits
with a double roller pump. The laser irradiated one
of the circuits (laser group), and none the other
(control group). In the laser group,
erythrocyte deformability and erythrocyte adenosine
triphosphate (ATP) levels were significantly
higher, and free hemoglobin levels were
significantly lower than those in the control group.
Subsequent morphological findings by means of
scanning electron microscope were consistent
with these results. Low power He-Ne laser protected
human erythrocytes in the preserved diluted whole
blood from the damage caused by experimental
artificial heart-lung machines. The clinical
application of low power laser treatment for
extracorporeal circulation is suggested.
Low-Intensity Near-Infrared
Laser Radiation-Induced Changes Of
Acetylcholinesterase
Activity Of Human Erythrocytes
Kujawa J, Zavodnik L, Zavodnik I, Bryszewska M.
Department of Rehabilitation, Medical University of
Lodz, Lodz, Poland.
jkujawa@box43.gnet.pl
J Clin Laser Med Surg. 2003 Dec;21(6):351-5.
OBJECTIVE: The aim of the present study was to
investigate the transformations of red
blood cells produced by low-intensity infrared laser
radiation (810 nm).
BACKGROUND DATA: Low-intensity (the output power of
a laser device in the milliwatt range)
laser radiation as a local phototherapeutic modality
is characterized by its ability to induce nonthermic,
nondestructive photobiological processes in cells
and tissues. However, the exact theory concerning
the therapeutic effects of laser biostimulation has
not been developed.
MATERIALS AND METHODS: The suspensions of human
erythrocytes in PBS (10% hematocrit) were irradiated
with near-infrared (810 nm) therapy laser at
different light doses (0-20 J) and light power (fluence
rate; 200 or 400 mW) at 37 degrees C.
As the parameters characterizing
the cell structural and functional changes membrane
acetylcholinesterase (AchEase) activity, the
membrane potential, the level of intracellular
glutathione, the level of products of membrane lipid
peroxidation, and the cell osmotic stability
were measured.
RESULTS: It was found that near-infrared
low-intensity laser radiation produced complex
biphasic dose-dependent changes of the parameters of
AchEase reaction in the dose-dependent
manner: at smaller doses of radiation (6 J) the
maximal reaction rate and Michaelis-Menten
constant value decreased, and at higher radiation
doses these parameters increased. No
significant changes of erythrocyte stability,
cellular redox state (reduced glutathione or lipid
peroxidation product levels), or cell membrane
electrochemical potential were observed.
CONCLUSION: Low-intensity
near-infrared laser radiation (810 mn) produced
AchEase activity
changes, reflecting the effect of light on the
enzyme due to energy absorption. Protein molecule
conformational transitions and enzyme activity
modifications in cells have been suggested as
laser radiation-induced events. |
